1、下载emboss
conda create -n emboss_env -c bioconda emboss -y
conda activate emboss_env
2、检查needleall工具是否存在
needleall -help
3、进入序列文件夹
cd /mnt/d/1CAAS/Lab/Heyubin/protein/GPD16
4、多序列全局比对
needleall -asequence /mnt/d/1CAAS/Lab/Heyubin/protein/GPD16/GPD16.fasta -bsequence /mnt/d/1CAAS/Lab/Heyubin/protein/GPD16/Cas12j.fasta -aformat3 simple -outfile 1results.txt
